5J45

Crystal structure of Shrub, fly ortholog of SNF7/CHMP4B

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	100
Detector technology	PIXEL
Collection date	2015-03-19
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	0.9792
Spacegroup name	P 43
Unit cell lengths	29.767, 29.767, 174.311
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	43.578 - 2.758
R-factor	0.2212
Rwork	0.216
R-free	0.26880
RMSD bond length	0.003
RMSD bond angle	0.583
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHENIX
Refinement software	PHENIX (dev_1951)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000
High resolution limit [Å]	2.758	2.758
Rmerge	0.090
Number of reflections	3869
<I/σ(I)>	15.9
Completeness [%]	98.1
Redundancy	3.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.5	293.15	17% v/v PEG10K, 0.1 M NH4CH3CO2, and 100 mM Bis-Tris pH 5.5