5J3W
Crystal structures reveal signaling states of a short blue light photoreceptor protein PpSB1-LOV (dark state)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID29 |
| Synchrotron site | ESRF |
| Beamline | ID29 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2014-02-08 |
| Detector | DECTRIS PILATUS 6M-F |
| Wavelength(s) | 0.96863 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 102.379, 71.086, 91.264 |
| Unit cell angles | 90.00, 91.08, 90.00 |
Refinement procedure
| Resolution | 45.001 - 2.550 |
| R-factor | 0.1947 |
| Rwork | 0.192 |
| R-free | 0.25460 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3sw1 |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.499 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | MOLREP |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 45.001 | 2.660 |
| High resolution limit [Å] | 2.550 | 2.550 |
| Rmerge | 0.049 | 0.911 |
| Number of reflections | 21326 | |
| <I/σ(I)> | 14.7 | 1.5 |
| Completeness [%] | 99.2 | 98.9 |
| Redundancy | 5 | 4.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 293 | Na-cacodylate, MgCl2, PEG 3000 |
