5J34
Isopropylmalate dehydrogenase K232M mutant
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-10-12 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.979 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 76.803, 76.820, 209.497 |
| Unit cell angles | 90.00, 90.22, 90.00 |
Refinement procedure
| Resolution | 38.410 - 1.827 |
| R-factor | 0.1664 |
| Rwork | 0.165 |
| R-free | 0.18550 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | R8W |
| RMSD bond length | 0.007 |
| RMSD bond angle | 0.996 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 38.500 | 1.860 |
| High resolution limit [Å] | 1.820 | 1.820 |
| Rmerge | 0.642 | |
| Number of reflections | 211778 | |
| <I/σ(I)> | 28.6 | 2 |
| Completeness [%] | 98.8 | 99.4 |
| Redundancy | 3.4 | 2.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 277 | 0.16 M ammonium sulfate, 0.07 M sodium acetate, 17.5% (v/v) PEG-4000, and 20% (v/v) glycerol |
