5J1P
Lassa virus L protein cap-snatching endonuclease. Bound to two manganese ions
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SOLEIL BEAMLINE PROXIMA 1 |
Synchrotron site | SOLEIL |
Beamline | PROXIMA 1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2011-09-20 |
Detector | DECTRIS PILATUS 300K |
Wavelength(s) | 0.976 |
Spacegroup name | P 41 21 2 |
Unit cell lengths | 51.420, 51.420, 144.180 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 48.430 - 2.360 |
R-factor | 0.20819 |
Rwork | 0.204 |
R-free | 0.28861 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5j1n |
RMSD bond length | 0.009 |
RMSD bond angle | 1.470 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0123) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 48.430 | 2.460 |
High resolution limit [Å] | 2.360 | 2.360 |
Rmerge | 0.064 | 1.208 |
Number of reflections | 8543 | |
<I/σ(I)> | 22 | |
Completeness [%] | 99.8 | |
Redundancy | 6.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7 | 297 | 2mM MnCl, 5mM DPBA, 5 mM MgCl chloride hexahydrate, 0.05 M HEPES-Na pH7 25 % (v/v) PEG MME 550 |