5J10

De novo design of protein homo-oligomers with modular hydrogen bond network-mediated specificity

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 8.2.2
Synchrotron site	ALS
Beamline	8.2.2
Temperature [K]	100
Detector technology	CCD
Collection date	2015-07-09
Detector	ADSC QUANTUM 315r
Wavelength(s)	1
Spacegroup name	P 21 21 21
Unit cell lengths	41.740, 42.120, 123.670
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	61.835 - 2.020
R-factor	0.2179
Rwork	0.216
R-free	0.25170
RMSD bond length	0.004
RMSD bond angle	0.653
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (dev_1616)

Data quality characteristics

	Overall
Low resolution limit [Å]	61.840
High resolution limit [Å]	2.020
Number of reflections	14812
<I/σ(I)>	14.7
Completeness [%]	99.2
Redundancy	6.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	0.1 M Citric acid pH 3.5, 2.0 M Ammonium sulfate