5J0G
Monomeric Human Cu,Zn Superoxide dismutase, loops IV and VII deleted, apo form, circular permutant P7/8
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | MAX II BEAMLINE I911-3 |
| Synchrotron site | MAX II |
| Beamline | I911-3 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2014-09-11 |
| Detector | MARRESEARCH |
| Wavelength(s) | 1 |
| Spacegroup name | P 65 |
| Unit cell lengths | 74.140, 74.140, 59.420 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 37.100 - 2.500 |
| R-factor | 0.1843 |
| Rwork | 0.182 |
| R-free | 0.23540 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4bcz |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.881 |
| Data reduction software | iMOSFLM |
| Data scaling software | Aimless |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.7.0032) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 37.100 | 2.610 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Rmerge | 0.097 | 0.661 |
| Number of reflections | 6519 | |
| <I/σ(I)> | 14.1 | |
| Completeness [%] | 100.0 | |
| Redundancy | 7.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 0.2 M di-ammonium hydrogen citrate, 20 % w/v PEG 3350 |
