5J0F
Monomeric Human Cu,Zn Superoxide dismutase, loops IV and VII deleted, apo form, circular permutant P4/5
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | MAX II BEAMLINE I911-3 |
| Synchrotron site | MAX II |
| Beamline | I911-3 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2013-11-01 |
| Detector | MARRESEARCH |
| Wavelength(s) | 1 |
| Spacegroup name | P 65 |
| Unit cell lengths | 71.720, 71.720, 69.510 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 46.360 - 1.250 |
| R-factor | 0.1313 |
| Rwork | 0.129 |
| R-free | 0.16960 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4bcz |
| RMSD bond length | 0.022 |
| RMSD bond angle | 2.004 |
| Data reduction software | iMOSFLM |
| Data scaling software | Aimless |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.7.0032) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 46.360 | 1.290 |
| High resolution limit [Å] | 1.250 | 1.250 |
| Rmerge | 0.039 | 0.563 |
| Number of reflections | 56396 | |
| <I/σ(I)> | 29.2 | |
| Completeness [%] | 100.0 | |
| Redundancy | 6.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | EVAPORATION | 293 | 0.17 M ammonium sulfate, 25.5 % w/v PEG 4K, 15 % v/v Glycerol |
