5IZW

Crystal structure of RNA editing specific factor of designer PLS-type PPR-9R protein

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL19U1
Synchrotron site	SSRF
Beamline	BL19U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2015-04-15
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.9785
Spacegroup name	P 41
Unit cell lengths	67.412, 67.412, 56.752
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	36.501 - 1.738
R-factor	0.1769
Rwork	0.175
R-free	0.20770
Structure solution method	SAD
RMSD bond length	0.011
RMSD bond angle	1.106
Data scaling software	HKL-3000
Phasing software	SHELXDE
Refinement software	PHENIX ((1.10.1_2155: ???))

Data quality characteristics

	Overall
Low resolution limit [Å]	45.000
High resolution limit [Å]	1.738
Number of reflections	26305
<I/σ(I)>	24.1
Completeness [%]	99.9
Redundancy	13.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293.15	Magnesium sulfate heptahydrate, 1,3-Bis[tris(hydroxymethyl)methylamino]propane, PEG 4000