5IZS
De novo design of protein homo-oligomers with modular hydrogen bond network-mediated specificity
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 8.2.1 |
| Synchrotron site | ALS |
| Beamline | 8.2.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2015-07-22 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 3 |
| Unit cell lengths | 72.670, 72.670, 88.240 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 51.237 - 2.360 |
| R-factor | 0.2465 |
| Rwork | 0.245 |
| R-free | 0.26880 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.628 |
| Data reduction software | xia2 |
| Data scaling software | xia2 |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 51.240 | |
| High resolution limit [Å] | 2.360 | 2.360 |
| Rmerge | 0.148 | 0.715 |
| Number of reflections | 21259 | |
| <I/σ(I)> | 12 | 2.4 |
| Completeness [%] | 99.0 | 99.8 |
| Redundancy | 6.4 | 6.4 |
| CC(1/2) | 0.900 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 278 | 0.2 M Sodium Fluoride, 0.1 M MES pH 5.5 , 20 % PEG 400 |
