5IZO
Bacillus NanoRNase A (H103A) + 2 divalent cations + PO4 at the active site
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-E |
| Synchrotron site | APS |
| Beamline | 24-ID-E |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-04-22 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.97918 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 50.652, 119.644, 124.135 |
| Unit cell angles | 90.00, 91.59, 90.00 |
Refinement procedure
| Resolution | 47.340 - 1.950 |
| R-factor | 0.214 |
| Rwork | 0.212 |
| R-free | 0.26700 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5j21 |
| RMSD bond length | 0.022 |
| RMSD bond angle | 1.899 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 47.340 | 2.020 |
| High resolution limit [Å] | 1.950 | 1.950 |
| Rmerge | 0.090 | |
| Number of reflections | 106608 | |
| <I/σ(I)> | 17.44 | 2.18 |
| Completeness [%] | 94.1 | 80 |
| Redundancy | 3.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 298.15 | PEG MME 2000, sodium acetate, ammonium acetate, glycerol |
