5IZL

The crystal structure of human eEFSec in complex with GDPCP

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 21-ID-F
Synchrotron site	APS
Beamline	21-ID-F
Temperature [K]	100
Detector technology	CCD
Collection date	2015-04-04
Detector	MARMOSAIC 225 mm CCD
Wavelength(s)	0.97872
Spacegroup name	C 2 2 21
Unit cell lengths	94.302, 113.242, 329.725
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	48.773 - 2.720
R-factor	0.2462
Rwork	0.244
R-free	0.28620
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5izm
RMSD bond length	0.014
RMSD bond angle	1.478
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX ((1.10.1_2155: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	49.000	2.800
High resolution limit [Å]	2.720	2.720
Number of reflections	46595
<I/σ(I)>	14.5
Completeness [%]	100.0
Redundancy	19.4
CC(1/2)	0.200

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		285	0.1 M HEPES pH 7.6, 0.25 M ammonium sulfate, 18% (w/v) PEG 3,350, 0.02 M glycine, 2% dextran sulfate