5IZK
The crystal structure of human eEFSec in complex with GDP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 21-ID-G |
| Synchrotron site | APS |
| Beamline | 21-ID-G |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2015-06-11 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 0.97857 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 58.691, 96.861, 125.408 |
| Unit cell angles | 90.00, 90.25, 90.00 |
Refinement procedure
| Resolution | 42.942 - 3.250 |
| R-factor | 0.2982 |
| Rwork | 0.296 |
| R-free | 0.33780 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5izl |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.961 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.10.1_2155: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 43.000 | 3.050 |
| High resolution limit [Å] | 3.000 | 3.000 |
| Number of reflections | 26147 | |
| <I/σ(I)> | 7.6 | |
| Completeness [%] | 92.4 | |
| Redundancy | 3.7 | |
| CC(1/2) | 0.270 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 285 | 0.1 M Tris pH 8.5, 0.2 M NaI, 0.2 M KI, 18% (w/v) PEG 3,350 |
