5IZH

Lassa virus L protein cap-snatching endonuclease. apo form

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID14-4
Synchrotron site	ESRF
Beamline	ID14-4
Temperature [K]	100
Detector technology	CCD
Collection date	2011-06-22
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.939
Spacegroup name	P 21 21 21
Unit cell lengths	58.750, 60.130, 132.780
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	66.390 - 1.850
R-factor	0.20816
Rwork	0.205
R-free	0.25931
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3jsb
RMSD bond length	0.012
RMSD bond angle	1.478
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	REFMAC (5.8.0103)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	66.400	1.890
High resolution limit [Å]	1.850	1.850
Rmerge	0.056	0.726
Number of reflections	40510
<I/σ(I)>	15.11
Completeness [%]	99.5
Redundancy	4.78

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7	293	citrate 0.191 M PEG 3350 4% 0.1 M Hepes pH7 0.1 M strontium chloride 2mM MnCl