5IZH
Lassa virus L protein cap-snatching endonuclease. apo form
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID14-4 |
Synchrotron site | ESRF |
Beamline | ID14-4 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2011-06-22 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.939 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 58.750, 60.130, 132.780 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 66.390 - 1.850 |
R-factor | 0.20816 |
Rwork | 0.205 |
R-free | 0.25931 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3jsb |
RMSD bond length | 0.012 |
RMSD bond angle | 1.478 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0103) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 66.400 | 1.890 |
High resolution limit [Å] | 1.850 | 1.850 |
Rmerge | 0.056 | 0.726 |
Number of reflections | 40510 | |
<I/σ(I)> | 15.11 | |
Completeness [%] | 99.5 | |
Redundancy | 4.78 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7 | 293 | citrate 0.191 M PEG 3350 4% 0.1 M Hepes pH7 0.1 M strontium chloride 2mM MnCl |