5IZD
Wild-type glyceraldehyde dehydrogenase from Thermoplasma acidophilum in complex with NADP
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | BESSY BEAMLINE 14.1 |
Synchrotron site | BESSY |
Beamline | 14.1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2015-01-31 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.918409 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 95.289, 152.353, 149.905 |
Unit cell angles | 90.00, 92.19, 90.00 |
Refinement procedure
Resolution | 48.100 - 2.050 |
R-factor | 0.17021 |
Rwork | 0.168 |
R-free | 0.20540 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3k2w |
RMSD bond length | 0.016 |
RMSD bond angle | 1.748 |
Data reduction software | XDS (November 3, 2014) |
Data scaling software | XDS |
Phasing software | BALBES (1.0.0.Nov_16_2011) |
Refinement software | REFMAC (5.8.0135) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 48.100 | 2.060 |
High resolution limit [Å] | 1.950 | 1.950 |
Rmerge | 0.092 | |
Number of reflections | 310836 | |
<I/σ(I)> | 14.38 | 1.98 |
Completeness [%] | 99.8 | 98.9 |
Redundancy | 6.84 | |
CC(1/2) | 0.999 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 295 | 20 mM glutamic acid (racemic), 20 mM glycine, 20 mM serine (racemic), 20 mM alanine (racemic), 20 mM lysine-HCl (racemic), 50 mM MOPS, 50 mM Sodium HEPES, pH 7.5; 20% Ethylene Glycol, 10% PEG 8000 |