5IZ1
Physcomitrella patens FBPase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 HF |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2015-02-17 |
| Detector | MAR scanner 345 mm plate |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 74.075, 84.803, 105.748 |
| Unit cell angles | 90.00, 104.59, 90.00 |
Refinement procedure
| Resolution | 102.340 - 3.000 |
| R-factor | 0.25711 |
| Rwork | 0.254 |
| R-free | 0.31508 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1d9q |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.399 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0073) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 104.000 |
| High resolution limit [Å] | 3.000 |
| Rmerge | 0.120 |
| Number of reflections | 21633 |
| <I/σ(I)> | 8.8 |
| Completeness [%] | 88.5 |
| Redundancy | 3.6 |
| CC(1/2) | 0.993 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5.5 | 293 | 25 % PEG 3350 0.1 M Bis-Tris |
