5IYX

Crystal structure of the Arabidopsis receptor kinase HAESA in complex with the peptide hormone IDA and the co-receptor SERK1

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X10SA
Synchrotron site	SLS
Beamline	X10SA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2014-08-25
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	1.000020
Spacegroup name	P 21 21 21
Unit cell lengths	74.507, 100.456, 142.759
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	47.380 - 2.430
R-factor	0.19478
Rwork	0.193
R-free	0.23435
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5ixo 4lsc
RMSD bond length	0.009
RMSD bond angle	1.410
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	REFMAC (5.8.0135)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.570
High resolution limit [Å]	2.430	2.430
Number of reflections	41023
<I/σ(I)>	16.1	1.96
Completeness [%]	99.4	96.4
Redundancy	9.8	9.3
CC(1/2)	0.999

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	4	298	18% PEG 8,000, 0.2 M MgCl2, 0.1 M citric acid pH 4.0