5IWW

Crystal structure of RNA editing factor of designer PLS-type PPR/9R protein in complex with MORF9/RIP9

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U
Synchrotron site	SSRF
Beamline	BL17U
Temperature [K]	100
Detector technology	CCD
Collection date	2016-03-07
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.9793
Spacegroup name	P 64
Unit cell lengths	103.190, 103.190, 149.150
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	51.595 - 2.650
R-factor	0.1822
Rwork	0.180
R-free	0.22080
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.008
RMSD bond angle	0.942
Phasing software	PHASER
Refinement software	PHENIX ((1.10.1_2155: ???))

Data quality characteristics

	Overall
Low resolution limit [Å]	53.000
High resolution limit [Å]	2.650
Number of reflections	25740
<I/σ(I)>	15.1
Completeness [%]	98.5
Redundancy	5.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	Magnesium sulfate heptahydrate, 1,3-Bis[tris(hydroxymethyl)methylamino]propane, Polyethylene glycol 4000