5IWE
E45Q mutant of phenazine biosynthesis protein PhzF in complex with (5R,6R)-6-azaniumyl-5-ethoxycyclohexa-1,3-diene-1-carboxylate
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | BESSY BEAMLINE 14.1 |
| Synchrotron site | BESSY |
| Beamline | 14.1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-07-07 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.91841 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 56.032, 56.032, 155.742 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 46.328 - 1.710 |
| R-factor | 0.1505 |
| Rwork | 0.149 |
| R-free | 0.17340 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1u1v |
| RMSD bond length | 0.007 |
| RMSD bond angle | 0.945 |
| Data reduction software | XDS (v.052) |
| Data scaling software | Aimless (0.5.17) |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.10.1_2155: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 77.870 | 1.740 |
| High resolution limit [Å] | 1.710 | 1.710 |
| Rmerge | 0.124 | 0.754 |
| Number of reflections | 31593 | |
| <I/σ(I)> | 8.9 | 1.9 |
| Completeness [%] | 99.9 | 99.9 |
| Redundancy | 5.2 | 4.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 4.6 | 293.15 | 0.085M sodium acetate pH 4.6, 0.17M ammonium acetate, 25.5% (w/v) PEG 4000, 15% (v/v) glycerol, temperature 293.15K, VAPOR DIFFUSION, SITTING DROP |
