5IW6

anti-CD20 monoclonal antibody Fc fragment

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL18U1
Synchrotron site	SSRF
Beamline	BL18U1
Temperature [K]	80
Detector technology	PIXEL
Collection date	2015-03-21
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	1
Spacegroup name	P 21 21 21
Unit cell lengths	49.450, 80.004, 139.324
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	50.000 - 2.340
R-factor	0.22611
Rwork	0.224
R-free	0.25882
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1l6x
RMSD bond length	0.010
RMSD bond angle	1.470
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	MOLREP
Refinement software	REFMAC (5.8.0049)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.380
High resolution limit [Å]	2.340	2.340
Rmerge	0.087
Number of reflections	24182
<I/σ(I)>	32.1	2.1
Completeness [%]	99.9	100
Redundancy	6.8	6.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7	291	PEG 12000, Na HEPES