5IW3
anti-CD20 monoclonal antibody Fc fragment
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U |
| Synchrotron site | SSRF |
| Beamline | BL17U |
| Temperature [K] | 80 |
| Detector technology | CCD |
| Collection date | 2014-04-12 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.97915 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 64.158, 143.096, 56.556 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 50.000 - 2.050 |
| R-factor | 0.19006 |
| Rwork | 0.189 |
| R-free | 0.20731 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1l6x |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.436 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0049) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.100 |
| High resolution limit [Å] | 2.050 | 2.050 |
| Number of reflections | 17005 | |
| <I/σ(I)> | 14 | 2.9 |
| Completeness [%] | 98.1 | 82.6 |
| Redundancy | 3.6 | 3.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 291 | 30% PEG 400, 0.1 M NaAc pH4.6, 0.1M CdSO4 |
