5IVN
BC2 nanobody in complex with the BC2 peptide tag
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | BESSY BEAMLINE 14.1 |
Synchrotron site | BESSY |
Beamline | 14.1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2014-09-19 |
Detector | DECTRIS PILATUS3 6M |
Wavelength(s) | 0.918409 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 106.010, 31.530, 35.880 |
Unit cell angles | 90.00, 107.54, 90.00 |
Refinement procedure
Resolution | 50.550 - 1.000 |
R-factor | 0.12908 |
Rwork | 0.128 |
R-free | 0.14961 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5ivo |
RMSD bond length | 0.022 |
RMSD bond angle | 1.549 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0135) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.550 | 1.030 |
High resolution limit [Å] | 1.000 | 1.000 |
Number of reflections | 61121 | |
<I/σ(I)> | 18.42 | |
Completeness [%] | 99.6 | |
Redundancy | 9.5 | |
CC(1/2) | 1.000 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 293 | MES/imidazole, 2-Methyl-2,4-pentanediol, PEG 1000, PEG 3350, DL-Glutamatic acid monohydrate, DL-Alanine, Glycine, DL-Lysine monohydrochloride, DL-Serine |