5IUX
GLIC-V135C bimane labelled X-ray structure
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SOLEIL BEAMLINE PROXIMA 1 |
| Synchrotron site | SOLEIL |
| Beamline | PROXIMA 1 |
| Temperature [K] | 190 |
| Detector technology | PIXEL |
| Collection date | 2014-09-25 |
| Detector | DECTRIS PILATUS 6M-F |
| Wavelength(s) | 0.98 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 182.034, 134.075, 159.945 |
| Unit cell angles | 90.00, 102.51, 90.00 |
Refinement procedure
| Resolution | 12.000 - 2.600 |
| R-factor | 0.2049 |
| Rwork | 0.203 |
| R-free | 0.23680 |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.140 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | REFMAC |
| Refinement software | BUSTER (2.10.2) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 12.000 |
| High resolution limit [Å] | 2.600 |
| Rmerge | 0.036 |
| Number of reflections | 113718 |
| <I/σ(I)> | 13.04 |
| Completeness [%] | 98.0 |
| Redundancy | 2 |
| CC(1/2) | 0.999 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4 | 293 | 12% PEG4K, 15% DMSO, 100 mM Acetate de Sodium pH4, 200 mM Sodium isothiocyanate, 2% DMSO |
