5IUU
Crystal Structure of Indole-3-acetaldehyde Dehydrogenase in Apo form
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2015-12-04 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.979 |
| Spacegroup name | P 2 21 21 |
| Unit cell lengths | 80.889, 109.068, 143.912 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 40.445 - 2.090 |
| R-factor | 0.1972 |
| Rwork | 0.197 |
| R-free | 0.22180 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5iuv |
| RMSD bond length | 0.008 |
| RMSD bond angle | 0.858 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.10.1_2155: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.450 | 2.130 |
| High resolution limit [Å] | 2.090 | 2.090 |
| Number of reflections | 75921 | |
| <I/σ(I)> | 34.9 | 2.05 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 7.3 | 6.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 277 | 10% PEG8000, 100 mM HEPES, 8% ethylene glycol |
