5IUF
Bacillus NanoRNase A active site mutant bound to pAp
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-E |
| Synchrotron site | APS |
| Beamline | 24-ID-E |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-04-22 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.97918 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 50.522, 121.259, 123.688 |
| Unit cell angles | 90.00, 91.70, 90.00 |
Refinement procedure
| Resolution | 46.620 - 1.950 |
| R-factor | 0.177 |
| Rwork | 0.175 |
| R-free | 0.21600 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5ipp |
| RMSD bond length | 0.019 |
| RMSD bond angle | 1.891 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0135) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 46.620 | 2.020 |
| High resolution limit [Å] | 1.950 | 1.950 |
| Rmerge | 0.085 | 0.470 |
| Number of reflections | 107136 | |
| <I/σ(I)> | 17.88 | |
| Completeness [%] | 98.1 | 95.2 |
| Redundancy | 3.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 298.15 | PEG MME 2000, sodium acetate, ammonium acetate, glycerol |
