5IU0
Rubisco from Arabidopsis thaliana
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID29 |
| Synchrotron site | ESRF |
| Beamline | ID29 |
| Temperature [K] | 173 |
| Detector technology | PIXEL |
| Collection date | 2015-10-05 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.978 |
| Spacegroup name | I 4 |
| Unit cell lengths | 111.860, 111.860, 197.710 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 49.428 - 1.499 |
| R-factor | 0.1448 |
| Rwork | 0.136 |
| R-free | 0.15240 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 8ruc |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.842 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.10_2155: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 49.430 | 1.540 |
| High resolution limit [Å] | 1.499 | 1.499 |
| Rmerge | 0.101 | 0.740 |
| Number of reflections | 192721 | |
| <I/σ(I)> | 8.94 | 1.82 |
| Completeness [%] | 99.6 | 98.4 |
| Redundancy | 5 | 5 |
| CC(1/2) | 0.994 | 0.439 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8 | 293 | 0.1M HEPEs pH 8, 0.1M NaCl, 20% PEG4000, 0.01M NaHCO3, 0.01M MgCl2 |
