5ITM
The structure of truncated histone-like protein
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSRRC BEAMLINE BL13C1 |
| Synchrotron site | NSRRC |
| Beamline | BL13C1 |
| Temperature [K] | 123 |
| Detector technology | CCD |
| Collection date | 2015-07-25 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.9760 |
| Spacegroup name | P 31 |
| Unit cell lengths | 49.333, 49.333, 82.466 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 27.490 - 1.400 |
| R-factor | 0.1696 |
| Rwork | 0.168 |
| R-free | 0.19740 |
| RMSD bond length | 0.023 |
| RMSD bond angle | 2.301 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0135) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 1.450 |
| High resolution limit [Å] | 1.400 | 1.400 |
| Number of reflections | 43748 | |
| <I/σ(I)> | 30.6 | |
| Completeness [%] | 99.2 | |
| Redundancy | 4.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 295 | 0.1 M Tris-Cl, pH 7.5, 40% PEG 400 |
