5ITH

TIA-1 RRM2 recognition of target oligonucleotide

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	AUSTRALIAN SYNCHROTRON BEAMLINE MX2
Synchrotron site	Australian Synchrotron
Beamline	MX2
Temperature [K]	100
Detector technology	CCD
Collection date	2013-03-28
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.71080
Spacegroup name	P 65
Unit cell lengths	45.430, 45.430, 84.900
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	22.974 - 2.310
R-factor	0.2293
Rwork	0.228
R-free	0.26050
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3bs9
RMSD bond length	0.005
RMSD bond angle	0.908
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHENIX
Refinement software	PHENIX (1.8.4_1496)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	22.974	2.393
High resolution limit [Å]	2.310	2.310
Number of reflections	4381
<I/σ(I)>	35.07	5.17
Completeness [%]	100.0	100
Redundancy	12.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8	277	PEG 1500, Malonate-Imidazole-Borate, pH 8