5IT8

High-resolution structure of the Escherichia coli ribosome

This is a non-PDB format compatible entry.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 8.3.1
Synchrotron site	ALS
Beamline	8.3.1
Temperature [K]	100
Detector technology	CCD
Collection date	2013-02-01
Detector	ADSC QUANTUM 315
Wavelength(s)	1
Spacegroup name	P 21 21 21
Unit cell lengths	211.920, 434.360, 623.170
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	47.194 - 3.120
Rwork	0.190
R-free	0.22000
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4ybb
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	BUSTER
Refinement software	BUSTER (2.11.6)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	47.194	8.940	3.380
High resolution limit [Å]	3.117	6.320	3.160
Rmerge	0.122	0.083	0.332
<I/σ(I)>	82.3	18.6	7.77
Completeness [%]	89.4	99.4	99.3
Redundancy	3.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	MICROBATCH	6.5	291	PEG8k, MPD