5ISY

Crystal structure of Nudix family protein with NAD

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL19U1
Synchrotron site	SSRF
Beamline	BL19U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2015-05-08
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.9786
Spacegroup name	P 21 21 21
Unit cell lengths	52.411, 98.702, 113.739
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	45.000 - 2.354
R-factor	0.1852
Rwork	0.182
R-free	0.24440
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2gb5
RMSD bond length	0.008
RMSD bond angle	1.009
Phasing software	PHASER
Refinement software	PHENIX ((1.10.1_2155: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	45.000	2.440
High resolution limit [Å]	2.350	2.350
Rmerge	0.057	0.187
Number of reflections	25141
<I/σ(I)>	25.69
Completeness [%]	99.4
Redundancy	6.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.5	291	PEG 3350, Sodium Malonate, Bis-Tris