5ISU

2.2 Angstrom Crystal Structure of ABC Transporter Substrate Binding Protein CtaP (Lmo0135) from Listeria monocytogenes.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 21-ID-G
Synchrotron site	APS
Beamline	21-ID-G
Temperature [K]	100
Detector technology	CCD
Collection date	2016-02-25
Detector	MARMOSAIC 300 mm CCD
Wavelength(s)	0.97856
Spacegroup name	P 1 21 1
Unit cell lengths	46.637, 75.160, 70.517
Unit cell angles	90.00, 99.54, 90.00

Refinement procedure

Resolution	29.100 - 2.200
R-factor	0.18005
Rwork	0.178
R-free	0.22315
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4oet
RMSD bond length	0.009
RMSD bond angle	1.434
Data reduction software	HKL-3000
Data scaling software	HKL-3000
Phasing software	MOLREP
Refinement software	REFMAC (5.8.0135)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	29.100	2.240
High resolution limit [Å]	2.200	2.200
Rmerge	0.050	0.756
Number of reflections	24855
<I/σ(I)>	24.5	2
Completeness [%]	99.8	100
Redundancy	3.7	3.8
CC(1/2)	0.629

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	292	Protein: 15.0 mg/ml, 0.1M Tris HCl (pH 8.3), 0.5mM Cysteine; Screen: Classics II (D8), 0.1M HEPES (pH 7.5), 25% (w/v) PEG3350.