5IRR

Crystal structure of Septin GTPase domain from Chlamydomonas reinhardtii

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I04
Synchrotron site	Diamond
Beamline	I04
Temperature [K]	100
Detector technology	PIXEL
Collection date	2014-04-26
Detector	DECTRIS PILATUS 2M
Wavelength(s)	0.9200
Spacegroup name	C 1 2 1
Unit cell lengths	177.580, 39.410, 130.900
Unit cell angles	90.00, 118.50, 90.00

Refinement procedure

Resolution	43.628 - 2.040
R-factor	0.1964
Rwork	0.195
R-free	0.23220
Structure solution method	SAD
RMSD bond length	0.003
RMSD bond angle	0.639
Data reduction software	xia2
Data scaling software	xia2
Phasing software	SHELX
Refinement software	PHENIX

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	43.630	43.630	2.090
High resolution limit [Å]	2.040	9.120	2.040
Rmerge	0.086	0.040	0.729
Number of reflections	49936
<I/σ(I)>	11.4
Completeness [%]	97.1	98	95.9
Redundancy	4.1	3.9	3.9
CC(1/2)	0.997

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8.5	291	12.5% PEG 1000, 12.5% PEG 3350, 12.5% MPD; 30mM of each sodium nitrate, disodium hydrogen phosphate, ammonium sulfate; 10mM bicine/Trizma base pH 8.5