5IRF
Reverse transcriptase domain of group II intron maturase from Roseburia intestinalis in P1 space group
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-E |
| Synchrotron site | APS |
| Beamline | 24-ID-E |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2015-10-15 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.97918 |
| Spacegroup name | P 1 |
| Unit cell lengths | 50.290, 79.160, 86.020 |
| Unit cell angles | 109.00, 90.56, 105.04 |
Refinement procedure
| Resolution | 46.470 - 1.600 |
| R-factor | 0.1924 |
| Rwork | 0.191 |
| R-free | 0.22660 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5hhj |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.784 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX ((dev_2254: ???)) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 46.470 |
| High resolution limit [Å] | 1.600 |
| Number of reflections | 152938 |
| <I/σ(I)> | 19.3 |
| Completeness [%] | 96.1 |
| Redundancy | 3.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 291.15 | 100 mM Bis-Tris propane pH 8.5, 200 mM NaOAc-3H2O, 20% PEG 3350 |
