5IQO
Crystal structure of the E. coli type 1 pilus subunit FimG (engineered variant with substitutions Q134E and S138E; N-terminal FimG residues 1-12 truncated) in complex with the donor strand peptide DsF_T4R-T6R-D13N
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06DA |
| Synchrotron site | SLS |
| Beamline | X06DA |
| Temperature [K] | 80 |
| Detector technology | PIXEL |
| Collection date | 2014-11-04 |
| Detector | DECTRIS PILATUS 2M-F |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 48.060, 61.850, 50.280 |
| Unit cell angles | 90.00, 109.12, 90.00 |
Refinement procedure
| Resolution | 45.409 - 1.302 |
| R-factor | 0.1414 |
| Rwork | 0.141 |
| R-free | 0.16960 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3bfq |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.167 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.500 |
| High resolution limit [Å] | 1.300 | 1.300 |
| Rmerge | 0.070 | 0.997 |
| Rmeas | 0.070 | 0.997 |
| Number of reflections | 67917 | |
| <I/σ(I)> | 13.9 | |
| Completeness [%] | 99.2 | |
| Redundancy | 6.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | MOPS-NaOH pH 7.6 (RT), PEG-1500, CoCl2, NaCl |
