5IQL

Crystal structure of YEATS2 YEATS bound to H3K27cr peptide

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U
Synchrotron site	SSRF
Beamline	BL17U
Temperature [K]	100
Detector technology	CCD
Collection date	2015-03-11
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.9791
Spacegroup name	I 4 2 2
Unit cell lengths	75.791, 75.791, 119.331
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	35.221 - 2.100
R-factor	0.2094
Rwork	0.204
R-free	0.25320
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4tmp
RMSD bond length	0.007
RMSD bond angle	0.842
Data scaling software	HKL-2000
Phasing software	MOLREP
Refinement software	PHENIX (1.10_2155)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	2.140
High resolution limit [Å]	2.100	5.700	2.100
Rmerge	0.082	0.051	0.736
Number of reflections	10507
<I/σ(I)>	12
Completeness [%]	99.9	98.8	100
Redundancy	11.4	10	12.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	7	291	0.1M Bis-Tris propane, 4.0 M NH4Ac, pH 7.0