5IQH
Aminoglycoside Phosphotransferase (2'')-Ia (CTD of AAC(6')-Ie/APH(2'')-Ia) S214A mutant in complex with GMPPNP and Magnesium
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | CLSI BEAMLINE 08ID-1 |
Synchrotron site | CLSI |
Beamline | 08ID-1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2014-02-25 |
Detector | RAYONIX MX-300 |
Wavelength(s) | 0.9795 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 90.220, 99.710, 93.280 |
Unit cell angles | 90.00, 105.26, 90.00 |
Refinement procedure
Resolution | 89.990 - 2.250 |
R-factor | 0.16091 |
Rwork | 0.159 |
R-free | 0.19910 |
Structure solution method | FOURIER SYNTHESIS |
Starting model (for MR) | 5byl |
RMSD bond length | 0.016 |
RMSD bond angle | 1.615 |
Data reduction software | iMOSFLM (0.1.27) |
Data scaling software | Aimless (0.3.11) |
Phasing software | REFMAC |
Refinement software | REFMAC (5.8.0073) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 89.990 | 2.300 |
High resolution limit [Å] | 2.250 | 2.250 |
Rmerge | 0.074 | 0.374 |
Number of reflections | 75692 | |
<I/σ(I)> | 10.6 | 2.4 |
Completeness [%] | 96.6 | 98.3 |
Redundancy | 2 | 1.9 |
CC(1/2) | 0.985 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 296 | 80-120mM MgCl2, 8% glycerol, 10% PEG 3350, 100mM HEPES pH 7.5 |