5IQA

Aminoglycoside Phosphotransferase (2'')-Ia (CTD of AAC(6')-Ie/APH(2'')-Ia) in complex with GMPPNP and Magnesium

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	CLSI BEAMLINE 08ID-1
Synchrotron site	CLSI
Beamline	08ID-1
Temperature [K]	100
Detector technology	CCD
Collection date	2013-05-05
Detector	RAYONIX MX-300
Wavelength(s)	0.9795
Spacegroup name	P 1 21 1
Unit cell lengths	90.170, 100.320, 94.060
Unit cell angles	90.00, 105.00, 90.00

Refinement procedure

Resolution	56.040 - 2.150
R-factor	0.16882
Rwork	0.167
R-free	0.20567
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3uzr
RMSD bond length	0.016
RMSD bond angle	1.562
Data reduction software	MOSFLM (0.1.27)
Data scaling software	Aimless (0.3.11)
Phasing software	PHASER (2.5.1)
Refinement software	REFMAC (5.8.0073)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	56.040	2.190
High resolution limit [Å]	2.150	2.150
Rmerge	0.108	1.211
Number of reflections	83016
<I/σ(I)>	7.8	1.4
Completeness [%]	94.4	100
Redundancy	4.7	4.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	296	80-120mM MgCl2, 8% glycerol, 10% PEG 3350, 100mM HEPES pH 7.5