5IQ7
Crystal structure of 10E8-S74W Fab in complex with an HIV-1 gp41 peptide.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-ID |
| Synchrotron site | APS |
| Beamline | 22-ID |
| Temperature [K] | 93 |
| Detector technology | CCD |
| Collection date | 2013-03-01 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 1.0000 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 135.762, 189.257, 71.717 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 37.512 - 3.287 |
| R-factor | 0.2328 |
| Rwork | 0.230 |
| R-free | 0.28200 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4g6f |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.685 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | PHENIX |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 3.340 |
| High resolution limit [Å] | 3.270 | 8.400 | 3.270 |
| Rmerge | 0.111 | 0.046 | 0.627 |
| Number of reflections | 14165 | ||
| <I/σ(I)> | 10.7 | ||
| Completeness [%] | 98.5 | 98.6 | 85.1 |
| Redundancy | 8.8 | 7.1 | 4.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 293 | 40% PEG400, 0.1M NaCitrate, 0.1M Tris-HCl, 7.5 |
