5IQ4
Crystal structure of RnTmm mutant Y207S soaking
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL19U1 |
| Synchrotron site | SSRF |
| Beamline | BL19U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-09-26 |
| Detector | PSI PILATUS 6M |
| Wavelength(s) | 0.98 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 72.969, 61.364, 104.706 |
| Unit cell angles | 90.00, 94.04, 90.00 |
Refinement procedure
| Resolution | 35.720 - 1.500 |
| R-factor | 0.159 |
| Rwork | 0.156 |
| R-free | 0.20600 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5ipy |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.081 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | HKL-3000 |
| Refinement software | PHENIX (1.6.4_486) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.530 |
| High resolution limit [Å] | 1.500 | 1.500 |
| Rmerge | 0.100 | |
| Number of reflections | 130501 | |
| <I/σ(I)> | 36.8 | |
| Completeness [%] | 88.2 | |
| Redundancy | 3.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | 0.2 M sodium acetate, 0.1 M Bis-Tris propane (pH 6.5) and 20% (w/v) PEG 3350. The Y207S soaking crystals were obtained using crystals of Y207S soaked with 15 mM TMA for 15 min. |
