5IPD
Human Histidine Triad Nucleotide Binding Protein 1 (hHint1) nucleoside thiophosphoramidate covalent intermediate complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 17-ID |
| Synchrotron site | APS |
| Beamline | 17-ID |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-04-18 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 1.000 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 78.445, 46.241, 64.218 |
| Unit cell angles | 90.00, 94.78, 90.00 |
Refinement procedure
| Resolution | 39.800 - 1.750 |
| R-factor | 0.159 |
| Rwork | 0.158 |
| R-free | 0.18800 |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.935 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | PHENIX |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 63.995 | 1.760 |
| High resolution limit [Å] | 1.750 | 1.750 |
| Rmerge | 0.063 | 0.353 |
| Number of reflections | 22992 | |
| <I/σ(I)> | 14.4 | |
| Completeness [%] | 98.6 | 97.3 |
| Redundancy | 3.2 | 3.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 6.4 | 293 | 100 mM MES, 34% PEG 8000 |
