5IP9
Structure of RNA Polymerase II-TFIIF complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2012-07-03 |
| Detector | PSI PILATUS 6M |
| Wavelength(s) | 0.91889 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 222.820, 392.730, 283.420 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 48.930 - 3.900 |
| R-factor | 0.1628 |
| Rwork | 0.162 |
| R-free | 0.19370 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3po2 |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.260 |
| Data scaling software | XSCALE |
| Refinement software | BUSTER-TNT (2.10.2) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.540 | |
| High resolution limit [Å] | 3.450 | 15.430 | 3.450 |
| Rmerge | 0.206 | 0.032 | 4.011 |
| Number of reflections | 112721 | ||
| <I/σ(I)> | 9.19 | 42.41 | 0.63 |
| Completeness [%] | 99.9 | 95.9 | 100 |
| Redundancy | 7.5 | ||
| CC(1/2) | 0.997 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 295 | peg 6000, sodium acetate, ammonium acetate, hepes, tcep |
