5IP8
Lambda-Ru(TAP)2dppz bound to d(CCGGCTCCGG)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I02 |
| Synchrotron site | Diamond |
| Beamline | I02 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2008-12-08 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.9795 |
| Spacegroup name | P 43 |
| Unit cell lengths | 47.450, 47.450, 35.130 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 23.730 - 1.760 |
| R-factor | 0.18949 |
| Rwork | 0.188 |
| R-free | 0.21957 |
| Structure solution method | SAD |
| RMSD bond length | 0.017 |
| RMSD bond angle | 2.692 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | SHELXDE |
| Refinement software | REFMAC (5.8.0135) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 23.730 | 1.810 |
| High resolution limit [Å] | 1.760 | 1.760 |
| Rmerge | 0.024 | 0.728 |
| Number of reflections | 7868 | |
| <I/σ(I)> | 29.8 | 2.2 |
| Completeness [%] | 99.8 | 100 |
| Redundancy | 6.5 | 6.4 |
| CC(1/2) | 0.999 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 291 | 6 uL of a solution containing 10% v/v MPD, 0.04M Sodium Cacodylate, 0.012M Spermine, 0.08M KCl, 0.02M BaCl2. 1uL 1mM DNA, 1uL 4mM rac-RuTAP2dppz |
