5IP7
Structure of RNA Polymerase II-Tfg1 peptide complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2013-07-13 |
| Detector | PSI PILATUS 6M |
| Wavelength(s) | 0.97972 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 221.220, 392.730, 282.190 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 48.930 - 3.520 |
| R-factor | 0.1542 |
| Rwork | 0.154 |
| R-free | 0.17970 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3po2 |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.290 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Refinement software | BUSTER-TNT (2.10.2) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.610 | |
| High resolution limit [Å] | 3.520 | 15.740 | 3.520 |
| Rmerge | 0.113 | 0.025 | 1.054 |
| Number of reflections | 294213 | ||
| <I/σ(I)> | 15.67 | 62.75 | 2 |
| Completeness [%] | 100.0 | 96.6 | 100 |
| Redundancy | 7.1 | ||
| CC(1/2) | 0.998 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 295 | PEG 6000, sodium acetate, ammonium acetate, hepes, tcep |
