5IP3

Tomato spotted wilt tospovirus nucleocapsid protein-ssDNA complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL41XU
Synchrotron site	SPring-8
Beamline	BL41XU
Temperature [K]	100
Detector technology	CCD
Collection date	2013-01-25
Detector	RAYONIX MX225HE
Wavelength(s)	1.0000
Spacegroup name	P 1 21 1
Unit cell lengths	68.940, 86.457, 66.322
Unit cell angles	90.00, 112.10, 90.00

Refinement procedure

Resolution	37.753 - 3.000
R-factor	0.2534
Rwork	0.251
R-free	0.29130
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4YBW
RMSD bond length	0.005
RMSD bond angle	0.938
Refinement software	PHENIX (dev_1426)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	47.000	47.035	3.180
High resolution limit [Å]	3.000	8.850	3.000
Rmerge	0.074	0.038	0.928
Rmeas	0.080	0.041	0.996
Total number of observations	110284
Number of reflections	14540	591	2273
<I/σ(I)>	17.11	45.1	2.38
Completeness [%]	99.4	97.8	98
Redundancy	7.58

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	298	0.1 M HEPES pH7.5, 25% (w/v) PEG1000