5IOO
Accommodation of massive sequence variation in Nanoarchaeota by the C-type lectin fold
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-E |
| Synchrotron site | APS |
| Beamline | 24-ID-E |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2014-11-17 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.979 |
| Spacegroup name | P 61 |
| Unit cell lengths | 143.998, 143.998, 59.261 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 71.999 - 2.521 |
| R-factor | 0.2064 |
| Rwork | 0.202 |
| R-free | 0.24980 |
| Structure solution method | SAD |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.266 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 71.999 | 2.610 |
| High resolution limit [Å] | 2.520 | 2.520 |
| Rmerge | 0.250 | 1.000 |
| Number of reflections | 46142 | |
| <I/σ(I)> | 12.5 | |
| Completeness [%] | 99.8 | |
| Redundancy | 7.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 293.15 | 100 mM HEPES (pH 7.5), 50 mM magnesium chloride, 30% PEG monoethyl ether 550 |
