5IOH
RepoMan-PP1a (protein phosphatase 1, alpha isoform) holoenzyme complex
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X25 |
Synchrotron site | NSLS |
Beamline | X25 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2013-11-12 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 1.1 |
Spacegroup name | P 21 21 2 |
Unit cell lengths | 160.769, 57.089, 73.635 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 46.545 - 2.566 |
R-factor | 0.1785 |
Rwork | 0.176 |
R-free | 0.21520 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4mov |
RMSD bond length | 0.002 |
RMSD bond angle | 0.610 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | PHASER |
Refinement software | PHENIX (1.8.4_1496) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.640 |
High resolution limit [Å] | 2.566 | 2.600 |
Rmerge | 0.017 | |
Number of reflections | 22441 | |
<I/σ(I)> | 15.3 | 2.4 |
Completeness [%] | 99.3 | 90.4 |
Redundancy | 11.5 | 5.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 4 | 277 | 100 mM Sodium Malonate, 12% PEG 3350 |