5INW
Structure of reaction loop cleaved lamprey angiotensinogen
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U |
| Synchrotron site | SSRF |
| Beamline | BL17U |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2015-04-10 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.97923 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 42.400, 119.360, 141.590 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 91.260 - 2.700 |
| R-factor | 0.20774 |
| Rwork | 0.204 |
| R-free | 0.27698 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3ndd |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.734 |
| Data reduction software | iMOSFLM |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0135) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 91.260 | 2.830 |
| High resolution limit [Å] | 2.700 | 2.700 |
| Rmerge | 0.095 | 1.065 |
| Number of reflections | 18674 | |
| <I/σ(I)> | 12.3 | 1.7 |
| Completeness [%] | 90.9 | 84.6 |
| Redundancy | 6.3 | 6.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | LIQUID DIFFUSION | 4.6 | 295 | 25% PEG 4000, 0.1M Na acetate pH4.6, 0.2M ammonium sulfate |
