5INR
A C69-family cysteine dipeptidase in complex with Ala-Pro from Lactobacillus farciminis
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL26B1 |
| Synchrotron site | SPring-8 |
| Beamline | BL26B1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2015-09-19 |
| Detector | RAYONIX MX225HE |
| Wavelength(s) | 1.00 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 167.398, 182.299, 98.335 |
| Unit cell angles | 90.00, 112.84, 90.00 |
Refinement procedure
| Resolution | 39.254 - 1.652 |
| R-factor | 0.16 |
| Rwork | 0.159 |
| R-free | 0.18220 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5iau |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.073 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 39.300 | 1.680 |
| High resolution limit [Å] | 1.650 | 1.650 |
| Rmerge | 0.058 | 0.388 |
| Number of reflections | 321818 | |
| <I/σ(I)> | 26.14 | 3.93 |
| Completeness [%] | 99.2 | 65.9 |
| Redundancy | 6.6 | 5.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 4.8 | 293 | 0.8 M 1,6-hexanediol, 10 mM CoCl2, 100 mM acetate buffer |
