5INB
RepoMan-PP1g (protein phosphatase 1, gamma isoform) holoenzyme complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL12-2 |
| Synchrotron site | SSRL |
| Beamline | BL12-2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-03-14 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.97950 |
| Spacegroup name | P 61 2 2 |
| Unit cell lengths | 86.371, 86.371, 215.894 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 37.400 - 1.300 |
| R-factor | 0.1334 |
| Rwork | 0.132 |
| R-free | 0.15340 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1jk7 |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.243 |
| Data reduction software | XDS (Nov 3, 2014) |
| Data scaling software | Aimless (Nov 3, 2014) |
| Phasing software | PHASER (2.5.6) |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 37.400 | 1.320 |
| High resolution limit [Å] | 1.300 | 1.300 |
| Rmerge | 0.049 | 0.093 |
| Number of reflections | 234338 | |
| <I/σ(I)> | 23.5 | 2.4 |
| Completeness [%] | 100.0 | 99.4 |
| Redundancy | 8.9 | 8.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 4.3 | 277 | 1 M Sodium Malonate |
