5IN1
Crystal Structure of the MRG701 chromodomain
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL19U1 |
| Synchrotron site | SSRF |
| Beamline | BL19U1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2016-03-06 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.987 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 31.786, 59.801, 40.616 |
| Unit cell angles | 90.00, 102.58, 90.00 |
Refinement procedure
| Resolution | 29.901 - 1.400 |
| R-factor | 0.1838 |
| Rwork | 0.182 |
| R-free | 0.20450 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4pli |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.049 |
| Data scaling software | HKL-3000 |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.8.4_1496) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 30.000 |
| High resolution limit [Å] | 1.400 |
| Number of reflections | 28872 |
| <I/σ(I)> | 29.05 |
| Completeness [%] | 98.6 |
| Redundancy | 5.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.5 | 290 | 0.1M Sodium acetate pH 4.5, 25% PEG 4000, 0.2M ammonium sulfate |
