5IMO

Nanobody targeting mouse Vsig4 in Spacegroup P3221

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL19U1
Synchrotron site	SSRF
Beamline	BL19U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2015-07-23
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.987
Spacegroup name	P 32 2 1
Unit cell lengths	49.906, 49.906, 183.720
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	43.220 - 2.100
R-factor	0.2452
Rwork	0.241
R-free	0.28130
RMSD bond length	0.005
RMSD bond angle	0.844
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall
Low resolution limit [Å]	43.220
High resolution limit [Å]	2.100
Number of reflections	16270
<I/σ(I)>	8.96
Completeness [%]	99.6
Redundancy	6.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	5.5	293	0.1M Sodium citrate, 20% PEG3000, pH 5.5